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2023 · Q22

A leaf was plucked from a plant on a sunny day and kept for 2 minutes in boiling water. It was subsequently immersed in boiling alcohol and treated with iodine solution. What will be the final colour of the leaf after the test?

General KnowledgeBiology
  1. AColourless
  2. BGreen
  3. CBlue
  4. DWhite

Explanation

This is the standard starch test: boiling water kills the leaf cells, boiling alcohol removes the chlorophyll (decolourising the leaf), and iodine solution then stains any starch present. Iodine forms a blue-black complex with starch, so a leaf photosynthesizing on a sunny day turns blue, not green, colourless or white.