CRISPR technology has rapidly advanced in recent years, with new tools and clinical breakthroughs. Highlight the recent developments in CRISPR-based gene editing technology applications. Discuss the opportunities and ethical challenges these advancements present for healthcare and society. (Answer in 250 words)

CRISPR technology used Cas9 as gene-scissor for gene-editing and modification as potent biotechnology. CRISPR Cas-9 Technology diagram: cut palindrome - CRISPR add new gene Recent development in CRISPR-Cas9: 1. More better identification and removed reduce dot to mutation 2. Small sequence modification possible now, e.g. only big e.g. 1cmR CRISPR at 2025 3. Targeted modification inmRNA also possible e.g. mRNA vaccine 4. Country-specific gene research supported through CRISPR Opportunities of CRISPR: 1. Gene-disease counter e.g. vaccinia 2. Low-cost compare to earlier TALEN 3. Faster vaccine development e.g. COVID moderna 4. Precise, so less escape of mutation 5. Children: disability reduction e.g. germline modification 6. Agriculture: lead to better variety, pest resistant 7. Country specific: gene research supported through CRISPR

Ethical Challenges: 1. Eugenicity: design baby - playing with evolution 2. Germline modification: kept against a 'Standfor ethic' 3. Gene-divide: as poor out a fit 4. Prompt: unwanted mutation in sampart use. Way-Ahead: 1. No germline modification (26mrc guidelines) 2. Low cost though subsidize 3. More RD spend it is for research e.g. only 0.7% 4. Cabinet of excellence for use in Agriculture e.g. RZRCK collaboration 5. Proper procedure by CRISPR

ethics e.g. Standfor ethics 3. Gene-divide: as poor out fit 4. Prompt: unwanted mutation in sampart use. Way-Ahead: 1. No germline modification (26mrc guidelines) 2. Low cost though subsidize 3. More RD spend it is for research e.g. only 0.7% 4. Cabinet of excellence for use in Agriculture e.g. RZRCK collaboration 5. Proper procedures by CRISPR