2026 · Q94
Consider the following stages of insulin production procedure through gene technology :
- 1Isolation of insulin coding gene from humans
- 2Inserting the insulin coding gene into a plasmid vector
- 3Inserting the recombinant plasmid vector into the bacterium (E. coli) Which of the following is a correct sequence of the above?
general-sciencebiotech-environment-science
- A1-2-3
- B1-3-2
- C3-2-1
- D2-3-1
Explanation
The standard rDNA procedure for human insulin is: first isolate the insulin-coding gene from human cells, then insert it into a plasmid vector to form recombinant DNA, and finally insert the recombinant plasmid into the bacterium E. coli for expression. The correct sequence is therefore 1-2-3, which is option A.